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CN
CAS:3681-93-4
Appearance:Light yellow to yellow powder
Storage:2-8℃
Purity:HPLC≥98%
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UHPLC
UHPLC system (Shimadzu, Japan) consisted of a model LC30AD pump and a model SIL-30AC autosampler with a Phenomenex Kinetex C18 column (100 mm × 2.1 mm, 1.7 μm).The chromatograph was equipped with a gradient mobile phase. Mobile phases were water with 0.1% of formic acid (A) and acetonitrile with 0.1% of formic acid (B). The gradient used was as follows: 0.01 min, 5% B; 0.01–3 min, 5–25% B; 3–5 min, 25–30% B; 5–12 min, 30–50% B; 12– 18 min, 50–100% B; 18–22 min, 100% B; 22–22.1 min, 100– 5% B; 22.1–27 min, 5% B. The injection volume of sample was 2 μL. The flow rate was 0.3 mL/min and the column temperature was 35℃. The Q-TOF-MS system (AB, America) with an ESI source was performed in positive mode and negative mode. The parameters of ESI–MS were set as follows: ion source gas 1 (50 psi), ion source gas 2 (50 psi), curtain gas (35 psi), temperature (500℃), ion spray voltage floating (5500 V), declustering potential (100 V), collision energy (10 eV).
References：
Ma T, Sun J, Li X, Ma Y, Liu L, Guo L, Liu Q, Sun Y. Optimization of extraction for Anemarrhena asphodeloides Bge. using silica gel-based vortex-homogenized matrix solid-phase dispersion and rapid identification of antioxidant substances. J Sep Sci. 2020 Jun;43(11):2180-2192. doi: 10.1002/jssc.202000101. Epub 2020 Mar 29. PMID: 32133730.
