EN
CN
CAS:501-98-4
Appearance:White to off-white powder
Storage:2-8℃
Purity:HPLC≥98%
Manual Download
HPLC
The quantification of p-coumaric acid and cinnamic acid was carried out using an HPLC system equipped with a DAD and an C18 column (250 mm × 4.6 mm, 5 μm). Quantification was carried out at 310 nm for p-coumaric acid and 280 nm for cinnamic acid. Gradient elution was carried out using water containing 0.2% phosphoric acid (A) and acetonitrile (B) with the following elution program: 0–8 min, 10%–20% B; 8–40 min, 20%–30% B; 40–60 min, and 30%–50% B. The flow rate was 1.0 mL min-1, and the injection volume was 20 μL.
References：
A S G , B Y M , B Y W ,et al.Effects of storage temperature on bisdemethoxycurcumin formation in fresh-cut yam ( Dioscorea opposita )[J].Journal of Food Composition and Analysis, 2021.DOI:10.1016/j.jfca.2021.104106.
HPLC–MS
We used an HPLC system, which consisted of a binary solvent delivery system, an on-line degasser, an auto-sampler, a column temperature controller, and a diode array detector. Separation was conducted using a C18 column (5 μm, 150 mm × 4.6 mm), and the temperature was set to 40℃. The injection volume was 0.01 mL, and the flow rate was 1 mL/min. Gradient elution was conducted using a binary system consisting of 10 % formic acid in DW (A) and 10 % formic acid in acetonitrile (B). The utilized gradient was as follows: isocratic 1 % B for 6 min, 1% – 3% B for 4 min, 3 % – 15 % B for 20 min, 15 % – 40 % B for 20 min, 40 % – 90 % B for 5 min, 90 % B isocratic for 10 min, 90 % – 1 % B for 5 min, and 1 % B isocratic for 10 min. A re-equilibration operation followed. The UV detection wavelength was set to 280 nm.The voltages of the sample cone and the capillary were set to 17 and 3000 V for the positive ion mode and 22 and 2000 V for the negative ion mode.
References：
[1]Zhang,Junhong,Tian,et al.Isolation and identification of phenolic compounds in Chinese purple yam and evaluation of antioxidant activity[J].LWT-Food Science & Technology, 2018, 96:161-165.
HPLC
HPLC conditions: chromatographic column was SB-C18 column (4.6 × 250 mm, 5 μm) and the column temperature was maintained at 30℃. The wavelength of ultraviolet detector were 280 nm and 320 nm, and the gradient elution with a flow rate at 0.8 mL/min was performed by mobile phase A (2% acetic acid in deionized water) and solvent B (methanol; 0–10 min, 5–30%; 10–35 min, 30–50%; 35–40 min, 50–60%; 40–45 min, 60–70%; 45–50 min, 70–5%; 50–55 min, 5%).
References：
Xu J, Qi Y, Zhang J, Liu M, Wei X, Fan M. Effect of reduced glutathione on the quality characteristics of apple wine during alcoholic fermentation. Food Chem. 2019 Dec 1;300:125130. doi: 10.1016/j.foodchem.2019.125130. Epub 2019 Jul 4. PMID: 31325746.
