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Determination of Significance：
Soil hydroxylamine reductase can reduce the intermediate product hydroxylamine formed in the process of soil nitrogen metabolism to ammonia, and the reduced compounds in the soil can be used as hydrogen donors. Its strength affects the ammonia volatilization loss of nitrogen in the process of soil nitrogen metabolism, and indirectly affects the utilization efficiency of nitrogen.
Measurement Principle：
Fe3+ in ammonium ferric sulfate can oxidize hydroxylamine to nitrogen and reduce itself to Fe2+, Fe2+ forms orange red complex with o-phenanthroline under weak acid condition, orange red complex has absorption peak at 510nm. hydroxylamine reductase acts on hydroxylamine that could reduce the amount of formation of complex, and the decrease of absorption value at 510nm can reflect the activity of hydroxylamine reductase.
Self Provided：
Spectrophotometer/ Microplate reader, scales, centrifuge, transferpettor, micro glass cuvette/ 96-well flat-bottom plate, vortex shaker, nitrogen blower, mortar, 30-50 mesh sieve and distilled water.
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