Determination of Significance：

Blood phosphorus mainly refers to the inorganic phosphorus in blood, which exists in the form of inorganic phosphorus salt. The concentration of calcium and phosphorus in plasma is closely related. When expressed in mg/dL, the product of the two ([Ca]×[P]) is 30～40. When ([Ca]×[P]) > 40, calcium and phosphorus are deposited in bone tissue in the form of bone salt. If ([Ca]×[P]) < 35, it will hinder the calcification of bone, even make the bone salt dissolve, and affect the osteogenic effect. The relative stability of blood calcium and phosphorus content depends on the relative balance of calcium and phosphorus absorption and excretion, calcium and decalcification metabolism. These balances are regulated by hormones such as vitamin D3, parathyroid hormone and calcitonin.

Measurement Principle：

After removing the organic phosphorus from serum, the inorganic phosphorus salt and ammonium molybdate reagent generate phosphomolybdic acid, which is blue after being reduced by ferrous sulfate and has light absorption at 660 nm.In this kit, the phosphorus content in blood is calculated by measuring the absorbance of 660 nm.

Self Provided：

Centrifuge, transferpettor, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate and distilled water.

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