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Determination of Significance：
Acyltransferases are a large family of multifunctional proteins, which are mainly responsible for catalyzing various acylation and deacylation reactions in the body, playing an important role in gene expression, metabolism and signaling.
Measurement Principle：
Acyltransferase catalyzes acetyl CoA to transfer acetyl to butanol, and at the same time reduces DTNB to generate TNB; TNB has an absorption peak at 412 nm, and the rate of increase in absorbance at 412 nm is measured to calculate Acyltransferase activity.
Self Provided：
Spectrophotometer/ microplate reader, analytical balance, micro quartz cuvette/ 96-well plate (UV), distilled water, centrifuge, water bath / constant temperature incubator, adjustable pipette, mortar/homogenizer, ice and distilled water
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