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Your Position: Home > Biochemical Assay Kits > Protease Series > Alkali Proteinase(AKP) Activity Assay Kit
Alkali Proteinase(AKP) Activity Assay Kit

Storage:2-8℃

  BC2305Manual
Alkali Proteinase(AKP) Activity Assay Kit
Cat.No：
BC2305
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC2305-100T/48S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

AKP is a serine protease, which catalyzes the hydrolysis of protein peptide bond in alkaline condition. In addition, the enzyme can hydrolyze ester bond and amide bond. It has the function of trans ester and trans peptide. The enzyme is one of the main industrial enzymes, which is widely used in pharmaceutical, silk, food, leather and other industries.

Measurement Principle：

In alkaline condition, AKP hydrolyzes casein to produce tyrosine. In alkaline condition, tyrosine reduced phosphomolybdic acid to tungsten blue. Tungsten blue has a characteristic absorption peak at 680 nm. The activity of AKP can be calculated by measuring the increasing rate of 680 nm absorbance.

Self Provided：

Mortar/homogenizer, desk centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, water bath, magnetic stirrer, transferpettor, 1.5 mL centrifuge tube and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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