Determination of Significance:
Fructose-1,6-diphosphate (FDP) is an important intermediate product in the glycolysis process. It can regulate a variety of enzymes, improve cell energy metabolism, increase energy utilization, anti-arrhythmia and anti-tissue peroxidation. FDP is widely used in clinical medicine.
Measurement Principle:
Aldolase catalyzes the cleavage of fructose 1,6-diphosphate. The product reacts with 2,4- dinitrophenylhydrazine in acid medium to form 2,4-dinitrophenylhydrazone, which is dark red in alkaline solution and has a characteristic absorption peak at 540 nm.
Self Provided:
spectrophotometer/Microplate reader, desk centrifuge, adjustable transferpettor, water bath /incubator, micro glass cuvette/ 96 well flat-bottom plate, mortar / homogenizer, ultrasonic crusher, ice and distilled water.
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