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Your Position: Home > Biochemical Assay Kits > Photosynthesis Series > Glyceraldehyde-3-phosphate Dehydrogenase(GAPDH) Activity Assay Kit
Glyceraldehyde-3-phosphate Dehydrogenase(GAPDH) Activity Assay Kit

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  BC2215Manual
Glyceraldehyde-3-phosphate Dehydrogenase(GAPDH) Activity Assay Kit
Cat.No：
BC2215
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC2215-100T/96S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

GAPDH (EC 1.2.1.12) catalyzes the oxidation of glyceraldehyde 3-phosphate to 1,3-diphosphoglyceride. It is the key enzyme of glycolysis pathway. It is closely related to the pathway of gluconeogenesis, the maintenance of blood glucose concentration and the occurrence of diabetes. It plays an important role in the disorders of glucose, lipid and protein metabolism.

Measurement Principle：

3-phosphoglycerate kinase can catalyze the production of 1,3-diphosphoglyceride from triphosphate and ATP. GAPDH reversely catalyzes the formation of glyceraldehyde-3-phosphate, inorganic phosphorus and NAD+ from 1,3-diphosphoglyceride and NADH. The decrease of NADH measured at 340 nm can reflect the activity of GAPDH.

Self Provided：

Desk centrifuge, ultraviolet spectrophotometer/microplate reader, constant temperature water bath, mortar/ homogenizer, micro quartz cuvette/96 well UV plate, transferpettor, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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