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Your Position: Home > Biochemical Assay Kits > Fatty Acid Metabolism Series > Total Cholesterol(TC) Content Assay Kit
Total Cholesterol(TC) Content Assay Kit

Storage:2-8℃

  BC1985Manual
Total Cholesterol(TC) Content Assay Kit
Cat.No：
BC1985
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC1985-100T/96S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

Total cholesterol (TC) is the sum of cholesterol contained in all lipoproteins, including free cholesterol and cholesteryl esters.

Measurement Principle：

The enzyme esterase catalyzes the hydrolysis of cholesteryl esters to produce free cholesterol (FC) and free fatty acids (FFA), thus converting cholesteryl esters to FC; furthermore, cholesteryl oxidase catalyzes the oxidation of FC to produce 4-Cholestenone and H2O2; finally, peroxidase catalyzes the oxidation of 4-aminoantipyrine and phenol by H2O2 to produce red quinones, which have a characteristic absorption peak at 500 nm. The color shade is proportional to the TC content.

Self Provided：

Spectrophotometer/microplate reader, balance, low temperature table centrifuge, water-bath, micro glass cuvette/96 well flat-bottom plate, pipette, EP tube, distilled water, isopropyl alcohol.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Total Cholesterol(TC) Content Assay Kit
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