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Your Position: Home > Biochemical Assay Kits > Amino Acid Metabolism series > Micro Glutamic-oxalacetic Transaminase（GOT）Assay Kit
Micro Glutamic-oxalacetic Transaminase（GOT）Assay Kit

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  BC1565Manual
Micro Glutamic-oxalacetic Transaminase（GOT）Assay Kit
Cat.No：
BC1565
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC1565-100T/48S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

GOT is widely found in animals, plants, microbes and cultured cells. It catalyzes the reversal of amino reactions and is an important enzyme in amino acid metabolism. In addition, GOT is the highest in cardiomyocytes and is commonly used as an assisted examination of myocardial infarction and myocarditis in clinical practice. The serum concentration of liver damage can also be increased.

Measurement Principle：

GOT catalyze α-ketoglutaric acid react with aspartate to produce glutamic acid and oxaloacetic acid. Oxaloacetic acid is further decarboxylated to form pyruvate, pyruvate can react with 2, 4-dinitrophenylhydrazine to produce 2,4-dinitrophenylhydrazone, which shows brownish red in alkaline condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.

Self Provided：

Spectrophotometer/microplate reader, water bath, desk centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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