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Determination of Significance：
Nitrite exists widely in water and soil, which is an important intermediate of organic nitrogen decomposition. It may cause cancer of digestive system if intake too much.
Measurement Principle：
In acidic condition, nitrite is reacted with aminobenzene sulfonic acid to form diazo-compound, then the compound is reacted with N-1-naphthylethylenediamine dihydrochloride to form purple-red azo compound, which can be detected by colorimetric assay at 540 nm.
Self Provided：
Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, centrifuge, balance, distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Soil Water Nitrite Content Assay Kit
