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Your Position: Home > Biochemical Assay Kits > Glutathione Series > γ-glutamylcysteine Ligase(GCL) Activity Assay Kit
γ-glutamylcysteine Ligase(GCL) Activity Assay Kit

Storage:-20℃

  BC1215Manual
γ-glutamylcysteine Ligase(GCL) Activity Assay Kit
Cat.No：
BC1215
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC1215-100T/48S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

GCL (Glutamylcysteineligase) is the rate limiting enzyme of GSH synthesis. GSH has feedback inhibition to GCL. GCL gene express manipulated by many factors, such as oxidants, antioxidants, growth factors and inflammatory factors. The activity of GCL has important influence to GSH content and ratio of GSH/GSSG.

Measurement Principle：

In the presence of ATP and Mg2+, GCL catalyzes the synthesis of γ-glutamyl cysteine from glutamate and cysteine, and Dephosphorylation of ATP produce inorganic phosphorus molecules. The activity of GCL can be calculated by measuring the increasing rate of inorganic phosphorus.

Self Provided：

Spectrophotometer/Microplate reader, Refrigerated centrifuge, water bath, adjustablepipette, micro glass cuvette/96 well flat-bottomplate, concentrated sulfuric acid and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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