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Your Position: Home > Biochemical Assay Kits > Glutathione Series > Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit
Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit

Storage:-20℃

  BC1155Manual
Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit
Cat.No：
BC1155
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC1155-100T/96S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

TrxR is a NADPH-dependent dimer selenase and includes FAD structure domain. TrxR belongs to pyridine nucleotide-disulfide REDOX enzyme, and form thioredoxin system with thioredoxin and NADPH. The activity of TrxR is similar with GR. TrxR could catalyzes GSSG reduce to GSH, which is the key enzyme in glutathione REDOX cycle.

Measurement Principle：

TrxR catalyzes NADPH to reduceDTNB form TNB and NADP+, TNB has a absorbance at 412 nm, but reduced glutathione reacts with DTNB to form TNB, so the 2-Vinylpyridine in this kit can inhibit reduced glutathione in sample, the activity of TrxR can be calculated by detecting increase rate of TNB at 412 nm.

Self Provided：

Spectrophotometer/microplate reader, ultra-micro glass cuvette/96 well flat-bottom plate,water bath, low temperature centrifuge, mortar/homogenizer, adjustable pipette,distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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