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Determination of Significance：
MDHAR catalyzes MDHA to form AsA, which plays an important role in ascorbic acid redox metabolism.
Measurement Principle：
NADH reduces MDHA to generate AsA and NAD+ under the conditions of MDHAR catalysis. NADH has a characteristic absorption peak at 340 nm, but NAD+ is not. The activity of MDHAR can be calculated by measuring the decrease rate of absorption at 340 nm.
Self Provided：
Mortar/homogenizer, ice, desk centrifuge, spectrophotometer/microplate reader, micro quartz cuvette/96 well flat-bottom UV plate, adjustable pipette and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
