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Determination of Significance：
Invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose, which is one of the key enzymes in sucrose metabolism of higher plants. According to the optimal pH, Ivr can be divided into acid invertase(AI) and neutral invertase(Ni). NI mainly exists in the cytoplasm and is responsible for decomposing sucrose into fructose and glucose.
Measurement Principle：
NI catalyzes the degradation of sucrose to produce reducing sugar, and further reacts with 3,5-dinitrosalicylic acid to form brownish red amino compound, which has a characteristic light absorption at 540 nm, and the increase rate of light absorption at 540 nm in a certain range is in direct proportion to NI activity. The activity of NI is calculated by the increasing rate of light absorption.
Self Provided：
Spectrophotometer, desktop centrifuge, water-bath, adjustable pipette, 1 mL glass cuvette, mortar/homogenizer, ice and distilled water.
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