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Your Position: Home > Biochemical Assay Kits > ATP Series > ATP Content Assay Kit
ATP Content Assay Kit

Storage:-20℃

  BC0305Manual
ATP Content Assay Kit
Cat.No：
BC0305
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC0305-100T/96S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

ATP (adenosine 5'-triphosphate) is found broadly in animals, plants, microorganisms and cultured cells, which is described as the energy currency in all living systems. Detecting the content of ATP and calculating the level of energy charge can reflect the state of energy metabolism.

Measurement Principle：

Hexokinase (HK) catalyzes the synthesis of glucose and ATP into 6-phosphate glucose. 6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of glucose 6-phosphate and NADP to form NADPH. NADPH has a characteristic absorption peak at 340 nm, and the absorbance ratio of NADPH is in direct proportion to contents of ATP.

Self Provided：

Spectrophotometer/microplate reader, water bath/constant temperature incubator, refrigerated centrifuge, transferpettor, micro quartz cuvette/96 well UV flat-bottom plate, ice, mortar/ homogenizer, distilled water and chloroform.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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