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Determination of Significance：
Soil phosphatase is an enzyme which catalyzes soil organic phosphate mineralization, the activity level influence the decomposition and transformation of organic phosphate and its bio-availability directly, which is the index of evaluating the direction and intensity of soil phosphorus bio-transformation. Soil phosphatase is influenced by the content of carbon, nitrogen, available phosphorus in the soil and pH, its divided into three types: acidic, neutral and alkaline according to the optimum pH.
Measurement Principle：
In alkaline condition, soil alkaline phosphatase (S-AKP/ALP) can catalyzes disodium phenyl phosphate to form phenol and disodium hydrogen phosphate, the activity of S-AKP/ALP can be calculated by detecting the content of phenol.
Self Provided：
Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, desk centrifuge, transferpettor, Water bath/constant temperature incubator, analytical balance, mythylbenzene (express delivery not allowed), alcohol, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
60个土壤需要几个土壤酸性磷酸酶试剂盒2023-07-20 20:58:00
Administrator您好，关于试剂盒规格（1）50T/48S：50T指能够做50次测定，48S指可以测试48个样品。如果每个样品重复测定3次，那么只能测定16个样品。其中有2次测定用于空白管或者 对照管。值得特别注意的是，50T/24S中由于每个样品测定都需要做对照，因此能够测定样品的数量仅为8个（假设做3次重复） （2）100T/96S：100T指能够做100次测定，96S指可以测定96个样品，如果每个样品重复测定3次，那么只能测定32个样品。其中有4次测定用于空白管或 者对照管。 注意由于瓶璧和移液器吸附等，通常要比理论测定次数少1~3次。其它规格的含义依次类推。值得特别注意的是，100T/48S中由于每个样品测 定都需要做对照，因此能够测定样品的数量仅为16个（假设做3次重复）。