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Determination of Significance：
Polyphenol oxidase (PPO) is mainly found in animals, plants, microorganisms and culture cells. PPO is a copper-contained oxidase that oxidizes monophenols and diphenols to produce quinones. It is closely related to fruit and vegetable processing, tea quality and tissue culture.
Measurement Principle：
PPO can catalyze o-dihydroxybenzene to produce quinones which has absorbance at 410 nm.
Self Provided：
Spectrophotometer/microplate reader, refrigerated centrifuge, water bath, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/ homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
煮沸的样本是用上清液进行煮沸吗？2023-05-23 17:31:15
Administrator您好，是的。
您好？植物叶片组织测ppo，0.1g样品是直接加提取液嘛？还是要怎么处理2022-09-14 11:19:24
Administrator称取约 0.1g 组织，加入 1mL 提取液进行冰浴匀浆。8000g 4℃离心 10 分钟，取上清，置冰上待测