NEB原装酶
在不同反应缓冲液的活性
NEBuffer 1.1:<10%
NEBuffer 2.1: 50%
NEBuffer 3.1: 100%
CutSmart Buffer: 25%
特性
重组酶、省时酶。
反应条件
NEBuffer 3.1,37℃。
热失活:65℃ 20 分钟。
浓度
10,000 和 50,000 units/ml。
甲基化敏感性
对哺乳动物基因组 DNA CpG 甲基化敏感。
注意事项
彻底消化超螺旋质粒需要的 Notl 酶量为消化线性 DNA 的 5 倍。
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.