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Determination of Significance：
Pepsin is secreted by major cells of the gastric mucosa which break down proteins in food into small peptides. It is generally used for the identification of low-acid nerve disease. chronic gastritis, chronic gastric dilatation, chronic duodenitis can also cause a decrease in pepsin secretion.
Measurement Principle：
Pepsin can catalyze the hydrolysis of hemoglobin form tyrosine, which has characteristic absorbance at 275 nm. The enzyme activity can be calculated by measuring the change of the absorbance.
Self Provided：
Ultraviolet spectrophotometer, desk centrifuge, transferpettor, water bath, 1 mL quartz cuvette, mortar/homogenizer, ice and distilled water.
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