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Determination of Significance：
Trypsin selectively hydrolyzes peptide chains made up of carboxyl groups of lysine or arginine in denatured proteins and is an important digestive enzyme. In addition, trypsin is also widely used as an adjunct to the treatment of local edema, hematoma and abscess produced by abscesses, hemothorax, surgical inflammation, ulcers and traumatic injuries, etc.
Measurement Principle：
Trypsin catalyzes the hydrolysis of the ester linkage of BAEE to produce BA. BA has an absorption peak at 253nm. The activity of trypsin can be calculated by measuring the rate of increase in absorbance at 253nm.
Self Provided：
Ultraviolet spectrophotometer, centrifuge, transferpettor, 1 mL quartz cuvette, water-bath, mortar/ homogenizer, ice and distilled water.
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