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人急性单核细胞白血病细胞;THP-1
人急性单核细胞白血病细胞;THP-1
Cat.No:
SCC-121812
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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贴壁细胞培养

细胞名称:人急性单核细胞白血病细胞;THP-1

形态特性单核细胞

生长特性悬浮生长

培养条件RPMI 1640 (w/o Hepes) + 10%FBS

传代方法维持细胞浓度在2×105~1×106 cells/ml2~3天换液1

冻存条件基础培养基+5%DMSO+20%FBS

特征特性该细胞可以吞噬乳胶颗粒和激活的红细胞,细胞膜和胞浆内均没有免疫球蛋白,表达C3RFcR;可受佛波酯TPA诱导向单核系方向分化;可作为转染宿主

细胞处理方法:

1. 细胞在培养瓶中培养至状态良好后灌满培养基运输,获得细胞后用酒精棉球擦拭瓶口消毒,然后在超净台中操作。

2. 将细胞转移至50mL的无菌离心管中,1000rpm离心5-10min,用完全培养基重悬细胞,适宜的密度分瓶培养。

特别注意:(如使用公共实验室或初次接触细胞培养,建议添加双抗培养)

1. 我们使用自产培养基及进口血清培养细胞,在您拿回细胞后,如想更换其它品牌培养基,请依照逐次替换的原则,先保留培养瓶中的培养基,多日多次代逐步更换,以减轻对细胞的刺激。

2. 如签收时出现培养瓶壁破裂,漏液等情况请及时拍照并联系售后。

3. 细胞任何售后问题,均需拍照存档并在2周之内及时联系客服。

相关产品及货号

RPMI 1640 (31800)                                

细胞冻存液(24800)                            

1×PBS缓冲液(pH7.2- 7.4)P1020

         0.25%胰蛋白酶-EDTA消化液(T1300) 

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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