Products
人胚肺成纤维细胞;HFL1
人胚肺成纤维细胞;HFL1
Cat.No:
SCC-120411
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
In stock In stock In stock In stock
Qty:
-
+
Order now

贴壁细胞培养

细胞名称人胚肺成纤维细胞;HFL1

形态特性成纤维细胞样

生长特性贴壁生长

培养条件 90% F12K  10%FBS

传代方法 1:4~1:6传代,每周换液2-3次

冻存条件细胞冻存液

细胞处理方法

1. 弃去培养液,加少量消化液润洗一次。

2. 弃去液体,再加入适量的消化液,25cm2细胞瓶可加入1-1.5ml消化液,75 cm2细胞瓶可加入3ml消化液。

3. 置室温或37℃放置至细胞变圆或细胞分散为单个细胞,不要摇动以免细胞成团。

4. 加入新鲜培养液,吹打混匀并加置新瓶中。

特别注意:(如使用公共实验室或初次接触细胞培养,建议添加双抗培养)

1. 我们使用自产培养基及进口血清培养细胞,在您拿回细胞后,如想更换其它品牌培养基,请依照逐次替换的原则,先保留培养瓶中的培养基,多日多次代逐步更换,以减轻对细胞的刺激。

2. 如签收时出现培养瓶壁破裂,漏液等情况请及时拍照并联系售后。

3. 细胞任何售后问题,均需拍照存档并在2周之内及时联系客服。

相关产品及货号:

高糖DMEM (12100)                              

细胞冻存液(24800)                            

1×PBS缓冲液(pH7.2- 7.4)(P1020)

0.25%胰蛋白酶-EDTA消化液(T1300) 

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Sorry, there is no more information.
SERVICE FOR SCIENTIFIC RESEARCH,RESPONSIBLE FOR LIFE
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist, Beijing, China
Tel: +86 010 50973105
Technical Support Email: service@solarbio.com
Purchaser Email: purchase@solarbio.com
Sales Email: sales@solarbio.com
Copyright © 2005-2021 Solarbio all rights reserved.
 ICP Filing Certificate京ICP备12051307号-7