人胚肺成纤维细胞;HFL1
贴壁细胞培养
细胞名称:人胚肺成纤维细胞;HFL1
形态特性:成纤维细胞样
生长特性:贴壁生长
培养条件: 90% F12K 10%FBS
传代方法: 1:4~1:6传代,每周换液2-3次
冻存条件:细胞冻存液
细胞处理方法:
1. 弃去培养液,加少量消化液润洗一次。
2. 弃去液体,再加入适量的消化液,25cm2细胞瓶可加入1-1.5ml消化液,75 cm2细胞瓶可加入3ml消化液。
3. 置室温或37℃放置至细胞变圆或细胞分散为单个细胞,不要摇动以免细胞成团。
4. 加入新鲜培养液,吹打混匀并加置新瓶中。
特别注意:(如使用公共实验室或初次接触细胞培养,建议添加双抗培养)
1. 我们使用自产培养基及进口血清培养细胞,在您拿回细胞后,如想更换其它品牌培养基,请依照逐次替换的原则,先保留培养瓶中的培养基,多日多次代逐步更换,以减轻对细胞的刺激。
2. 如签收时出现培养瓶壁破裂,漏液等情况请及时拍照并联系售后。
3. 细胞任何售后问题,均需拍照存档并在2周之内及时联系客服。
相关产品及货号:
高糖DMEM (12100)
细胞冻存液(24800)
1×PBS缓冲液(pH7.2- 7.4)(P1020)
0.25%胰蛋白酶-EDTA消化液(T1300)
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.