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Determination of Significance：
XOD (EC 1.17.3.2) catalyzes the oxidation of hypoxanthine to xanthine and superoxide anion, which is one of the main sources of active oxygen and is also one of the key enzymes of nucleotide metabolism. XOD is mainly distributed in mammalian heart, lung, liver and other tissues. When liver function impaired, XOD is released into serum in a large amount, which has specific significance for the diagnosis of liver damage.
Measurement Principle：
XOD catalyzes hypoxanthine to produce xanthine and superoxide anion. Superoxide anion reacts with hydroxylamine hydrochloride to form NO2-, and the NO2- under the action of p-aminobenzenesulfonamide and naphthalene ethylenediamine hydrochloride is produced a red azo compound with a characteristic absorption peak at 530 nm. The color depth is linear with XOD activity.
Self Provided：
Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, centrifuge, micro glass cuvette/96-well flat-bottom plate, adjustable transferpettor, mortar/homogenizer, ice and distilled water.
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请问微量法和分光光度法哪个更准确？还有elisa法呢2022-10-06 21:51:25
Administrator您好，分光法和微量法原理相同，设计的不同大小的反应体系，分别适配比色皿和酶标板检测的，分光法更大的反应体系检测读数稳定性受误差影响要更小，微量法优势在于酶标仪能快速对多个样本完成检测。ELISA方法检测从原理上说检测灵敏度要比生化法灵敏度高，但会受到种属来源限定，生化法是不限制种属来源的，能否检测到取决于物质含量活性。
请问你们家的黄嘌呤氧化还原酶试剂盒，测溶血的血清会有影响吗2022-07-18 10:48:25
Administrator该试剂盒反应生成物显示紫红色，在530nm处检测其吸收峰。溶血的血清因血红蛋白颜色会影响最终吸光值检测，导致数据不准确。不建议使用。