Product Introduction

The kit uses the third generation RNA extraction technology, which can efficiently extract high purity and high quality total RNA from various animal tissues. Provide efficient DNA-cleaning Colunm, which can easily separate and adsorb genomic DNA from supernatant and tissue lysate, which is easy to operate and save time; The RNA-only Column can bind RNA efficiently and with a unique formulation, it can handle a large number of samples at the same time. The whole system RNase-Free, so that the extracted RNA does not degrade; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA protein, no DNA, no ion, no organic compound pollution.

Product Features

The whole process is operated at room temperature (15-25 ℃), without ice bath and low temperature centrifugation.

The kit is completely RNase-Free without the need for RNA degradation.

Dna-cleaning Column specifically binds DNA so that the kit can remove genomic DNA contamination without adding additional DNase.

High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.

Fast: Easy to operate, can be completed in 30 minutes

Safety: No organic reagent extraction required.

High quality: The extracted RNA fragments are of high purity, free of protein and other impurities, and can meet various subsequent experiments.

RNA application

The Total RNA extracted from the Animal Total RNA Isolation Kit can be used for various downstream molecular experiments, such as: cDNA synthesis, RT-PCR, Real Time PCR, Northern Blot, Dot Blot, in vitro translation, chip analysis, PolyA screening, molecular cloning and RNase protection analysis, etc.

RNA fragment storage

RNase-Free ddH2O is recommended for RNA eluting for immediate use in downstream experiments or storage at -80℃. At -80℃, RNA can be stored for one year.

Kit Component Information

Buffer RL1: Provides the environment needed for grinding and cracking of animal tissues.

Buffer RL2: Provides a specific upper column environment for RNA.

Buffer RW1: Removes proteins, DNA and other impurities from RNA.

Buffer RW2: Removes residual salt ions from RNA.

RNase-Free ddH2O: elution and purification of total RNA on the column membrane.

Dna-cleaning Column: Specific adsorption of DNA in tissue cleavage products, and filtration to remove solid impurities in cleavage products.

Rna-only Column: Specific adsorption of total RNA in the filtrate passing through the DNA-Cleaning Column.

Precautions

All experimental procedures are performed at room temperature (15-25 ℃) (including centrifugation), do not use ice bath and low temperature (4 ℃) centrifugation. The sample should avoid repeated freezing and thawing, otherwise the extracted RNA will degrade and the extraction amount will decrease. If the extracted RNA is used to clone full-length cDNA, 10μL β-mercaptoethanol is added to every 1 mL Buffer RL1, and it is recommended to use it on the spot. Buffer RL1 can be placed at 4℃ for 1 month after adding β-mercaptoethanol. If the extracted RNA is only used for other downstream operations such as qPCR or sequencing analysis, it can be chosen not to add β-mercaptoethanol, which will not affect the extraction effect. Before using the kit, add anhydrous ethanol to Buffer RL2 and Buffer RW2. RNA yield and quality are dependent on the amount of tissue sample and elution volume, and it is recommended to use 10-20 mg of tissue per 500 μL Buffer RL1. Elution volume: Elution liquid volume should not be less than 50 μL, otherwise it will affect RNA production. Please check the Buffer RL1 and Buffer RW1 in the kit for crystal precipitation. If the crystal precipitates after storage at low temperature, the Buffer can be placed at room temperature or 37℃ for a period of time, and the crystal is dissolved and mixed before use.

Self-provided reagent

Anhydrous ethanol; β-mercaptoethanol (optional)