Product Introduction

RNaseR is derived from E.coli and expressed through recombinant gene engineering. This enzyme is Mg2+ dependent 3 '-5' ribonuclease. It can digest all linear RNAs substrates, but cannot digest circularRNA, lariatRNA, double-stranded RNA, and 3 'protrusions. 7nt double-stranded RNA. This enzyme can efficiently remove linear RNA without secondary structure, thereby purifying circular RNA molecules for subsequent RNA-sequencing experiments.

Application

1. Enrichment of circular RNA in biological samples

2. Analysis and identification of intron lasso sequences

3. Circular RNA research

4. Variable splicing research

Precautions

(1) In the digestion of totalRNA, due to the presence of Rnas with more secondary structures, such as tRNA, rRNA, dsRNA, etc., the digestion activity of RNaseR on such substrates is significantly decreased. At this time, the digestion time should be extended to 2h, and RNaseR should be added at 1h for digestion. The reaction temperature is raised to 45℃if necessary to open RNA molecules containing secondary structures.

(2) For RNAs containing highlystructured, RNaseR is still not fully digested, and HaiGene will later introduce a 5 '-3' exonuclidenase to solve such problems.

(3) According to other reports, in RNAs containing highlystructured, the reaction volume can be amplified to 50μl, which can reduce the effect of secondary structure

Promote the degradation of RNA.

(4) High temperature and prolonged incubation may lead to non-enzymatic cleavage of RNA (H+ attack of water molecules on diester bonds). Therefore, digestion time and reaction temperature need to be optimized and adjusted according to the specific experiment.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.