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Lambda exonuclease

Storage:-20℃

Lambda exonuclease
Cat.No:
Q3050
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
Q3050-1000U Inquiry Inquiry Inquiry Inquiry
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Product Introduction

Lambda exonuclease acts on double-stranded DNA, gradually cutting off 5′ single nucleotides in the 5′→3′ direction. The optimal substrate is 5′ phosphorylated double-stranded DNA, which also slowly degrades single-stranded DNA and non-phosphorylated substrates. The enzyme cannot start digestion from cuts or gaps in DNA.

Precautions

(1) 1×LambdaExoBuffer: 67 mmglycine-KOH (pH9.4 25℃), 2.5mMMgCl2, 50μg/mlBSA, incubated at 37℃.

(2) The optimum reaction temperature of this enzyme is 37℃, and it can be inactivated at 75℃for 10min.

(3) The cutting speed of the 5′-0H end is 20 times slower than that of the 5′-PO4 end, and single-stranded DNA is 100 times slower than double-stranded DNA.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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