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Your Position: Home > Molecular Biology > Nucleic Acid Extraction/Purification > T7 Endonuclease I
T7 Endonuclease I

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T7 Endonuclease I
Cat.No：
Q3010
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
Q3010-250U Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Product Introduction

T7 endonuclease I recognizes and cuts incomplete paired DNA, cross-structured DNA, Holliday structured or crossed DNA, heterodouble-stranded DNA or, at a slower rate, double-stranded DNA with incisations.

This enzyme cuts the first, second, or third phosphodiester at the mismatched base 5′ end.

This product is a high purity protein obtained by cloning recombinant expression of T7 endonuclease I gene, which is not contaminated by other endonuclease or exonuclease.

Application

1. Detection of mutants formed by gene mutation, SNP, TALEN or CRISPR/Cas9

2. Identify mismatched DNA

3. Break down the four-way cross DNA or branch DNA

4. Detect or cut heterodimer DNA and cut DNA

5. Randomly cut linear DNA for shoot-gun cloning

Precautions

(1) 1×T7ExoBuffer: 50mMKAc, 20mMTris-Ac, 10mM

Mg(Ac) 2,1 mMDTT (pH7.9 25℃), incubated at 25℃.

(2) The optimal reaction temperature of the enzyme is 25℃, and it can be inactivated for 20min at 75℃.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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