Total protein extraction kit can extract total protein from a variety of bacteria, including gram-positive and Gram-negative bacteria, which can be used for crude preparation of purified protein and total protein preparation. The extraction process is simple and convenient. The kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents protease from degrading the protein and provides a guarantee for extracting high-quality protein. The protein extracted from the kit can be used for WesternBlotting, protein electrophoresis and other downstream protein research experiments. The protein extracted by this kit is a protein that has no natural activity. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

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