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Product Introduction:
Gentamicin sulfate salt is a broad-spectrum aminoglycoside antibiotic that works well against a large number of gram-negative and gram-positive bacteria, including some β-lactam sensitive vancomycin resistant (VRE) enterococcus, and some superbugs that are resistant to glycopeptide antibiotics. The bacteriostatic mechanism is that by binding to the 30S subunit of the bacterial ribosome, codon errors are caused, and the transport of peptidyl-trNA from the A site to the P site is blocked, thus inhibiting the synthesis of bacterial proteins.
Description:
Gentamicin can bind to 30S ribosomal subunits to cause code misreading and block the transfer of peptidyl-trNA from the acceptor site to the donor site. Gentamicin can combine with the outer membrane of Pseudomonas cell membrane, displace the existing cations, make the membrane unstable, and form holes on the cell surface, thus playing a bactericidal effect.
Use and synthesis method:
Gentamicin sulfate is widely used in the laboratory:
1) Microbiology research, often used in drug sensitivity tests to screen sensitive bacteria;
2) Inhibiting bacterial contamination in cell culture with an effective concentration of 0.5-50 μg/mL;
3) Molecular biology studies, commonly used to selectively screen positive clones carrying gentamicin resistance genes, usually using a concentration of 15μg/ml.
Physical shape:
Moisture (%) : ≤18.0
pH (4%, water) 25℃ : 3.5-5.5
Solubility:
Soluble in water, 50mg/ml as mother liquor, mother liquor should be at -20℃
Note :
(1) For cell experiments, after dissolution, disposable needle filter must be used to remove bacteria.
(2) For your safety and health, please wear a lab coat and wear disposable gloves.
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