Product Introduction:

Kanamycin sulfate is an aminoglycoside antibiotic that inhibits both gram-positive and Gram-negative bacteria and mycoplasma. The mechanism of action is to target binding to the bacterial 70S ribosome subunit, thereby inhibiting ribosome translocation and causing miscoding to further interfere with protein synthesis. Kanamycin sulfate can be used to inhibit bacterial contamination in cell culture.

Description:

This product is a solution of Kanamycin dissolved in double steaming water. It should be filtered and removed before use.

Thaw completely before use, thoroughly mix; This solution is generally used at a concentration of 100ug/ml, and is mixed according to the amount of 100ul (1/1000) added to 100ml medium. If pouring the medium plate, make sure the medium is not hot before adding.

Use and synthesis method:

In molecular biology studies, it is commonly used to select bacterial clones that successfully transform kanamycin resistance gene (kan gene); It is also commonly used in Agrobacterium-mediated transformation experiments to selectively screen plant tissues carrying the npt II (APH3) gene.

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