Product Introduction:

The Coomasylan G-250 dye is bound to the protein in an acidic solution so that the position of the dye's maximum absorption peak (Imax) changes from 465nm to 595nm, and the absorbance value A595 measured is proportional to the protein concentration within a certain concentration range. The protein concentration determined by Bradford method is not affected by the chemical substances in most samples. The concentration of merhydryl ethanol in the sample can be as high as 1M, and the concentration of dithithreitol can be as high as 5mM. However, affected by a slightly high concentration of detergent, it is necessary to ensure that the concentration of SDS is less than 0.1%, and that of Triton X-100 is less than 0.1%. The Tween 20, 60 and 80 were below 0.06%. For samples containing detergents, the BCA protein concentration assay kit produced by Solarbio is recommended.

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