The Lowry method consists of two steps: the first step is the reaction of protein with copper to form protein-copper complex under alkaline conditions; The second step is that this complex reduces the Folin reagent to produce a dark blue color, the shade of which is proportional to the protein content. The quantification range was 5 ~ 500μg/ml protein. The color reaction of Folin reagent is caused by tyrosine, tryptophan and cysteine, so if the sample contains phenols, citric acid and sulfhydryl compounds, it will interfere with the reaction. In addition, the color intensity of different proteins is slightly different due to the different contents of tyrosine and tryptophan.

Lowry method is free from the interference of lipid substances and is suitable for the determination of samples with high lipid content. Also tolerates considerable concentrations of detergents such as SDS.

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