RIPA buffer_Lysate_Protein Extraction/Quantification_Protein and Immunity

SolarbioHome

English

中文

0 My Cart

+86 010 50973105

Products

Biochemical Reagents

Antibiotics (Biochemical Reagents) Vitamins Carbohydrates and Derivatives Animal & Plant Hormones Surfactants Amino Acids & Peptides & Protein Enzymes & Coenzymes & Inhibitors Bases & Nucleic Acids & Derivatives Acids & Salts & Amines Biochemical Reagents Building Blocks PEG Derivatives

Protein and Immunity

Protein Extraction/Quantification Protein Electrophoresis Other Reagents Immunotechnical Services Recombinant Protein Whole Cell Protein

Small Molecule Compounds

Star Products Inhibitors & Antagonists & Agonists Peptides PROTAC Anti-Infection Plant Hormones Amino Acids & Derivatives Vitamins & Derivatives Carbohydrates & It's Analogues Cosolvent & Solubilizer Dye & Probe Reaction Substrate & Physicochemical Detection

Antibodies

Primary Antibodies Secondary Antibodies Loading Control Antibodies Label Antibodies Antigens Plant Antibodies Viral Antibodies Bacterial Antibodies Small Molecule Antibodies RNA Antibodies

ELISA

Human Rats Mice Other Species Antibody Pairs Self-Prepare Reagents

Biochemical Assay Kits

Micromethod Macromethod HPLC Anti-Reverse Series Oxidation Series Antioxidant Series Coenzyme Ⅰ Series Coenzyme Ⅱ Series Glycolysis Series Glutathione Series Vitamin C Metabolism Series ATP Series Nitrogen Metabolism Series Amino Acid Metabolism series Esterase Series Mitochondrial Respiratory Chain Series Tricarboxylic Acid Cycle Series Protease Series Fatty Acid Metabolism Series Starch Series Sucrose Series Sugar Metabolism Series Ion Series Soil Series Signal Series Transhydrogenase Series Other Series Protein quantification Pectin Series Photosynthesis Series Gluconeogenesis Series Vitamin Series

Standard References

Star Products Control Herbs Standard Solution CP Standards Imported Standards Standard References Derivatization Reagent Ion Pair Reagents Domestic Standard Material UV Spectroscopy Standards

Dye Reagents

Stain Solution Fixation Solution Dye

Cell Biology

Cell Detection Reagents Cell Growth Factor Cell Line Cell Culture Cell Isolation & Digestion Cell Staining & Probe Cell Transfection Limulus Lysate

Molecular Biology

Nucleic Acid Extraction/Purification PCR Nucleic Acid Electrophoresis Recombinant Clone Expression Molecular Hybridization

Chromatography Media

Fillers Prepacked Column

Microbial Culture

Strains Drug Sensi-Disc Culture Medium Others

Instruments & Consumables

Consumables Imported Consumables Pipette Instruments Consignment Products

Universal Buffer

Fermentation Products

Diagnostic Reagents

Magnetic Beads

Purified Magnetic Beads Immunoprecipitation Base Magnetic Beads

Nanomaterials

Graphite/Graphene Carbon Nanomaterials Molecular Sieve Other Nanomaterials Inorganic Nanomaterials Metallic Nanomaterial Graphenoid

Peptides

Substance P & Related Peptides Pituitary Adenylate Cyclase Activating Peptides Bag Cell Peptides Cholecystokinin RGD/RAD Peptides Opioid Peptides Cancer Research Apelin/APLN Viral-Associated Peptides Laminin Peptides Pituitary & Hypothalamic Hormone Melanocyte-Stimulating Hormone/MSH Adrenocorticotropic Hormone/ACTH Growth-Promoting Hormone Neuropeptides Thymopoietin & Thymosin Peptides Endothelin & It's Analogues Protein Kinases & Related Peptides Amyloid Peptides Multiple Sclerosis Peptide Tag Osteoporosis Research Matrix Metalloproteinase Substrate Hormones Calcitonin Antimicrobial Peptides Antioxidant Peptides Insect Neuropeptides Casein Morphine Peptides Enzyme Substrate Peptides Prolactin-Releasing Peptides Enkephalin Brain Natriuretic Peptides Endorphin & Endorphin Thrombin-Related Peptides Others Dynorphin Neurokinin Neuropeptides & Related Peptides Renal Myelin Peptides Growth Hormone & Growth Factor Somatostatin & It's Analogues Leptin Fragment Peptides Bombesin Gastrin Cell-Penetrating Peptides Fibronectin & Extracellular Matrix Peptides Anti-Inflammatory Peptides Natriuretic Peptides A Angiotensinogen Substrates & Inhibitors Angiopoietin & Angiotensin Bradykinin Thrombospondin Hematology Inflammation Research Allatostatins Inhibitor-Related Peptides Cosmetic Peptides

Your Position: Home > Protein and Immunity > Protein Extraction/Quantification > Lysate > RIPA buffer

RIPA buffer

Storage:Store PMSF at -20℃, lysis buffer at 4℃, valid for 1 year.

R0020Manual

RIPA buffer

Cat.No：

R0020

Brand：

Solarbio

SKU	Tongzhou Beijing	Haidian Beijing	Wuhan	Guangzhou
R0020-100ml	Inquiry	Inquiry	Inquiry	Inquiry

Qty：

Add to cart

Order now

Product Information Related Products Related Paper COA Technical Q&A

Product Description: RIPA tissue/cell lysate is the use of surfactants, etc., to cleave cell membranes (including nuclear membranes). The protein extracted by this reagent is soluble total protein.

Instruction:

If RIPA is found to have precipitation, please let it sit at room temperature for half an hour or in a room temperature water bath to dissolve the precipitation.

According to the amount used, add 10ul PMSF for every 1ml RIPA, so that the final concentration of PMSF is 1mM, mix and set aside (add PMSF now).

1. Sample pretreatment:

a) For adherent cells: Remove the culture medium and wash it again with PBS, normal saline or serum-free culture medium. The lysate was added at the ratio of 150-250 ul lysate per cell volume of the 6-well plate. Blow the gun a few times to make full contact between the lysate and the cells.

b) For suspended cells: Centrifuge the cells and add 150-250ul of lysate according to the number of cells in each well of the 6-well plate. Tap the suspended cells with your fingers to fully lysate them. There should be no obvious cell precipitation after full lysis. If the number of cells is large, it must be divided into 5-10×105 cells/tubes, and then cracked.

c) For tissue samples: Cut the tissue into fine fragments. Add the lysate at a ratio of 150-250ul per 20mg of tissue

(If the cleavage is insufficient, more lysate can be appropriately added, and if a high concentration of protein samples is required, the amount of lysate can be appropriately reduced).

Homogenize with a glass homogenizer until fully cracked.

2. Post-processing:

Centrifuge 10000-14000g of the cracked sample for 3-5 minutes, take the supernatant, and then perform subsequent PAGE, Western and immunoprecipitation operations.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：

These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

Related Paper

A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer

English Name	RIPA buffer
Unit	Bottle
Storage	Store PMSF at -20℃, lysis buffer at 4℃, valid for 1 year.
Specification	100ml