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DiI-Labeled High Density Lipoprotein, Human

NO.:H8910
Quantity: 3

Application

Lipid metabolism

Description

High Density Lipoprotein labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate.
Concentration: >1mg/ml(protein). Buffer containing phosphate-buffered saline at pH 7.4 and 0.01 mM EDTA.
Specifications: 0.22 micron membrane filtered, aseptically filled. Cell Culture Tested.
Absorbance Ratio: DiI/Protein=484nm/275nm=1.21
Storage:
This product is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C (Do not free). HDL products have a natural tendency to aggregate. Aggregates of this product can interfere with its use.  To clarify these aggregates out, simply spin in centrifuge tube  for 2 minutes.
Product Preparation:
Purified  HDL is labeled with the fluorescent probe, DiI, and reisolated by ultracentrifugation (1.063-1.21g/ml). The resultant product is exhaustively dialyzed against phosphate buffered saline, (pH 7.4), sterilized by membrane filtration and then aseptically packaged in a solution containing phosphate-buffered saline at pH 7.4 and 0.2 µM EDTA. Each lot is evaluated on a murine macrophage cell line for fluorescence uptake.