Anti-ALYREF Polyclonal Antibody

Conjugation :

Unconjugate

Host :

Rabbit

Clonality :

Polyclonal Antibody

Gene symbol :

ALYREF

Immunogen :

A synthetic peptide of human ALYREF

Synonym :

ALY;ALY/REF;BEF;REF;THOC4;THO complex subunit 4

Isotype :

IgG

Gene ID :

10189

Clone No :

Swiss Port :

Q86V81

Molecular weight :

27kDa

Purification :

Affinity purification

Reactivity :

Human Mouse Rat

Application :

WB IHC

Recommended dilution :

WB 1:5000-8000. IHC 1:100-300.

Subcellular location :

Nucleus Cytoplasm

Storage :

Store at -20°C. Avoid freeze / thaw cycles.

Background :

Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.