PRODUCT DESCRIPTION
Description :
Recombinant Human Platelet Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln28-Lys601 is expressed with a Fc tag at the C-terminus.Formulation :
Lyophilized from a 0.2 米m filtered solution of PBS, pH7.4.Purity :
Greater than 95% as determined by reducing SDS-PAGE.Endotoxin :
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.Bio Activity:Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 3.5 pg/ml.Reconstitution :
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 米g/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage :
Lyophilized protein should be stored at < -20~C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7~C for 2-7 days.Aliquots of reconstituted samples are stable at < -20~C for 3 months.Background :
Semaphorin-4G is the least characterized of the seven known Class 4 transmembrane semaphorin glycoproteins. Class 4 semaphorins play multiple roles in cell attraction or repulsion, such as development of nerve pathways in the brain, promoting or inhibiting proliferation, in some cases organizing immune cell interactions. Semaphorin-4G can be expressed early in development in the central and peripheral nervous systems and in sensory ograns, such as cochlea, olfactory epithelium, vomeronasal organ and retina. In adults, Semaphorin-4G can be found in liver,kidney and brain. The human Semaphorin-4G precursor consists of a 17 amino acids signal sequence, a 658 amino acids extracellular domain, a 21 amino acids transmembrane domain, a 142 amino acids cytoplasmic domain with one Ser/Thr phosphorylation site.RELATED PRODUCTS
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REFERENCE LIBRARY
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