Recombinant Human Protocadherin-10

Description :

Recombinant Human Protocadherin-10 is produced by our Mammalian expression system and the target gene encoding Gln19-Thr715 (Leu405Pro) is expressed with a 6His tag at the C-terminus.

Formulation :

Lyophilized from a 0.2 米m filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Purity :

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin :

Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is typically 113.2 ng/ml in the presence of 500 ng/mL of Recombinant Human IL-22.

Reconstitution :

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 米g/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage :

Lyophilized protein should be stored at < -20～C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7～C for 2-7 days.Aliquots of reconstituted samples are stable at < -20～C for 3 months.

Background :

Protocadherin-10 (PCDH10) is a single-pass type I membrane protein that contains six extracellular cadherin domains, one transmembrane domain and one cytoplasmic tail differing from those of the classical cadherins. As a potential calcium-dependent cell-adhesion neuronal receptor, it may plays a role in the establishment and function of specific cell-cell connections in the brain. PCDH10 moderately expressed in all regions of the brain examined, as well as in testis and ovary, and low expression in all other tested tissues.