Anti-PCNA Polyclonal Antibody

Conjugation :

Unconjugate

Host :

Rabbit

Clonality :

Polyclonal Antibody

Gene symbol :

PCNA

Immunogen :

A synthetic peptide of human PCNA

Synonym :

ATLD2;cb16;Cyclin;HGCN872;MGC8367

Isotype :

IgG

Gene ID :

5111

Clone No :

Swiss Port :

P12004

Molecular weight :

29kDa

Purification :

Affinity purification

Reactivity :

Human Mouse Rat

Application :

WB

Recommended dilution :

WB 1:500-2000.

Subcellular location :

Nucleus

Storage :

Store at -20℃. Avoid freeze / thaw cycles.

Background :

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.