Anti-KEAP1 Polyclonal Antibody

Conjugation :

Unconjugation

Host :

Rabbit

Clonality :

Polyclonal Antibody

Gene symbol :

KEAP1

Immunogen :

A synthetic peptide of Human KEAP1

Synonym :

INrf2;KLHL19;kelch-like ECH-associated protein 1

Isotype :

IgG

Gene ID :

9817

Clone No :

Swiss Port :

Q14145

Molecular weight :

69kDa

Purification :

Affinity purification

Reactivity :

Human Mouse Rat Monkey

Application :

WB

Recommended dilution :

WB 1:1000-3000.

Subcellular location :

Nucleus Cytoplasm

Storage :

Store at -20～C. Avoid freeze / thaw cycles.

Background :

Substrate-specific adapter of a BCR E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation. The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome.