Glutamic-pyruvic Transaminase(GPT) Activity Assay Kit

No :   BC1550
Size :   
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Detection Device :

Microplate reader

Application :

GPT is widely found in animals, plants, microbes and cultured cells, which is an important enzyme in amino acid metabolism. It catalyzes the transamination of amino acid and keto acid. In addition, GPT activity is very high in Mammalian liver cells. GPT is released into the blood when liver cells necrotic, serum GPT activity is significantly increased. Therefore, GPT is recommended as the most sensitive indicator of liver damage by the World Health Organization.
GPT catalyzes α-ketoglutaric acid and alanine aspartate into glutamic acid and oxaloacetic acid. Oxaloacetic acid is further decarboxylated to form pyruvate, pyruvate can react with 2, 4-dinitrophenylhydrazine to produce 2,4-dinitrophenylhydrazone, which shows brownish red in alkaline condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.
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