Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit

No :   BC1155
Size :   
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Detection Device :

Microplate reader

Application :

TrxR is a NADPH-dependent dimer selenase and includes FAD structure domain. TrxR belongs to pyridine nucleotide-disulfide REDOX enzyme, and form thioredoxin system with thioredoxin and NADPH. The activity of TrxR is similar with GR. TrxR could catalyzes GSSG reduct to GSH, which is the key enzyme in glutathione REDOX cycle.
TrxR catalyzes NADPH to reduct DTNB form TNB and NADP+, TNB has absorbance at 412 nm, but reduced glutathione reacts with DTNB to form TNB, so the 2-Vinylpyridine in this kit can inhibit reduced glutathione in sample, the activity of TrxR can be calculated by detecting increase rate of TNB at 412 nm.
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