NADP Malic Enzyme(NADP-ME) Activity Assay Kit

No :   BC1125
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PRODUCT DESCRIPTION

Detection Device :

Microplate reader

Application :

ME is widely exist in microbe, culturing cells, animal and plant endochylema. Especially it have high activity in plant tissue. ME catalyze the reversible reaction of malic acid oxidative decarboxylation to produce pyruvic acid and CO2 with the reduction of NAD(P)+, which is the crucial enzyme of malic acid metabolism. The activity of ME is related with biosynthesis and antioxidation. In recent years, the determination of plant ME activity become a hot spot in antioxidant research. According coenzyme specificity and substrate specificity, ME is divided into NAD-ME(EC1.1.1.38) and NADP-ME(EC1.1.1.40).
NADP-ME catalyze the reduction of NADP+ to NADPH, detect the increase rate of NADPH at 340 nm.
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