NAD Kinase(NADK) Activity Assay Kit

Detection Device :

Microplate reader

Application :

NADK (EC 2.7.1.23) is widely found in animals, plants, microorganisms and cultured cells. It is the only enzyme that can catalyze the phosphorylation of NAD+ to NADP+ in organisms. It can catalyze the phosphorylation reaction of NAD(H) with ATP or inorganic polyphosphate [poly(P)] as a phosphoryl donor to generate NADP(H). Therefore, NAD kinase plays an important role in the synthesis of NADP(H) and the regulation of the balance between NAD(H) and NADP(H).
NADK catalyzes the phosphorylation of NAD+ to produce NADP+; NADP+ can be reduced to NADPH by glucose-6-phosphate dehydrogenase; NADPH can reduce oxidized thiazole blue (MTT) through the dehydrogenation of PMS, and the activity of NADK can be reflected by its absorbance value at 570 nm.

Product Description :