Lacate Dehydrogenase(LDH) Activity Assay Kit

Detection Device :

Microplate reader

Application :

Lactate dehydrogenase (LDH or LD) is the terminal enzyme of the glycolysis pathway which is found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back.
NAD+ and lactic acid is oxidized to pyruvic acid by the catalysis of LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrophenylhydrazone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.

Product Description :