Pyruvate Kinase(PK) Activity Assay Kit

Detection Device :

HPLC

Application :

PK (EC 2.7.1.40) widely exists in animals, plants, microorganisms and cultured cells. It could catalyze the final step of the glycolysis process. PK is one of the major rate-limiting enzymes in the glycolysis process and one of the key enzymes for ATP production. Therefore, the determination of PK activity is of great significance.
PK catalyzes the generation of ATP and pyruvate from phosphoenolpyruvate and ADP. Lactate dehydrogenase further catalyzed NADH and pyruvate to generate lactic acid and NAD+. The NADH degradation rate was measured at 340 nm to reflect the activity of PK.

Product Description :