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Monkey TNF alpha Elisa Kit

Long Name: Tumor Necrosis Factor alpha
NO.:SEKMY-0001
Quantity: 11

Information

Assay Type: Solid Phase Sandwich ELISA
Sensitivity: 6 pg/ml
Detection Range: 31-2000 pg/ml
Assay Length: 2.5h
Sample Type: Serum; Plasma; Cell culture supernatant.
Synonyms: TNF; DIF; TNF-alpha; TNFA; TNFSF2; Tumour necrosis factor; Tumour necrosis factor alpha; TNF-┒; Tumor necrosis factor; TNLG1F

Background

Tumor necrosis factor alpha (TNF-┒), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-┒ is produced by a wide variety of immune and epithelial cell types. Human TNF-┒ consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-┒ shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-┒. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.
Cleavage of membrane bound TNF-┒ by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-┒. TNF-┒ trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-┒ regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-┒ is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.