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Prestained Protein Marker(14.4kD-97.4kD)

NO.:PR1600
Quantity: 10

Application

Description

Product Description:

The product contains 6 pre-stained standard protein of known molecular weight, each content is about 40ug, the original molecular weight range is 14.4kD-97.4kD, the molecular weight will change slightly after pre-dyeing.


The product with 6 pre-stained proteins covering a wide range molecular weights for 14.4kD-97.4kD. Each protein content is about 40ugThe molecular has small change after pre-staining which can directly observe the state of protein electrophoresis and to clearly judge the transmembrane effect of Western Blot. Six blue bands can be obtained after SDS-PAGE gel electrophoresis.

Instruction:

1. After opening, add 200 μl of 1× protein loading buffer, heat in a boiling water bath for 3-5 minutes, and dispensed into 10 μl per tube, store at -20 ° C, and take one tube at a time.

2. After long term preserved, preheat the tube 3-5min before electrophoresis.

3. Suggest the concentration of separating gel is 12%.

Components

Molecular weight(kDa)

Pre-stain molecular weight(kDa)

Phosphorylase B from rabbit muscle

97.4

99.0

Albumin bovine serum

66.2

70.0

Actin from rabbit muscle

43.0

43.0

Carbonic Anhydrase from bovine

31.0

31.0

Human Growth Hormone

22.0

24.0

Lysozyme of egg white

14.4

15.0


  • Prestained Protein Marker(43kD-200kD)

  • Tris-Glycine Running Buffer,5×

  • SDS-PAGE loading buffer,4×(with DTT)

  • WB Transfer Buffer,10×

  • SDS-PAGE Gel Kit

  • Bradford Protein Assay Kit