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TUNEL Apoptosis Assay Kit

NO.:T2190
Quantity: 10

Application

Apoptosis detection

Description

Kit Components:
Component A: 100X TF3-dUTP 1 vial (25 uL)
Component B: Reaction Buffer 1 bottle (5 mL)
Component C: 1000X Hoechst 1 vial (50 uL)
Introduction:
DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are secondarily labeled with a marker. All the existing TUNEL assays contain the highly toxic sodium cacodylate which might induces apoptosis and also decrease DNA production and DNA strands. Our TUNEL Apoptosis Assay Kit uses proprietary buffer system free of sodium cacodylate. The kit is based on incorporation of a fluorescence dye TF3 modified deoxyuridine 5'-triphosphates (TF3-dUTP) at the 3' OH ends of the DNA fragments that form during apoptosis. The assay is optimized for the direct detection of apoptosis in either detached or attached cells without using antibody.
The kit provides all the essential components with an optimized assay protocol. It is suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected at Ex/Em = 550nm/590 nm.