• sales@solarbio.com
  • Toll Free: 010-56371202
  • Mon - Fri : 9:00 -17:00

Oxidized Low Density Lipoprotein, Human

NO.:H7950
Quantity: 3

Application

Lipid metabolism

Description

Concentration: >2mg/ml protein. Buffer containing phosphate-buffered saline at pH 7.4 and 0.2 mM EDTA-Na2.
Purity: 98%, is determined agarose gel electrophoresis.
Specifications: 0.22 micron membrane filtered, aseptically filled. Cell Culture Tested.
Storage:
This product is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C (Do not freeze). After prolonged storage, some precipitate may be observed. This is normal for this product. Clarify out the aggregates by spinning in centrifuge tube for 2 minutes.
Biological Activity:
Sample lots of Solarbio Oxidized LDL are evaluated for receptor binding to peritoneal macrophages in conjunction with our Dil-Ox-LDL and [I-125]Ox-LDL
Preparation:
Human LDL(which was purified to homogeneity via ultra-centrifugation (1.019-1.063g/cc)) is oxidized using 5μM Cu2SO4(oxidant)in PBS at 37℃ for 20 hours. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of Ox-LDL migrates 2.0 fold further than the native LDL. This type Oxidized LDL is widely used to investigate lipid metabolism, but not induce cell apoptosis. Apoptosis inducement please choose High Oxidized LDL(Cat#H7980).